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Step 1: Open the SoftMax Pro IMAP FP protocol. x. • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. #4TI-0655) Microplates. Two different agonists, acetylcholine and carbachol, were tested. Spectranet customer portalThe microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. Select Yes at the warning. Extended linear dynamic range. Shaking: Linear, orbital, and double orbital. Assay setup for a 384-well low-volume plate. SLS & DLS: 2 µL quartz cuvettes 45 µL quartz cuvettes 4 mL generic glass cuvette DLS only:Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 8 mm distance between well bottoms to plate bottom. x. FlexIII. #N207) LAL Reagent Water (Lonza cat. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. Streamline your workflow with comparative confluence assays to identify and verify gene edits. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans. For a higher . Tecan’s quality control and validation packages help our customers to fulfill all regulatory requirements for their Tecan microplate readers in an efficient, cost-effective way. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Microplate Readers. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax Plus 384 Microplate Reader (Molecular Devices cat. DynaPro ™ Plate Reader III. 305 reference level saturation. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. If you have an xml plate object, then. Scan every well . XML file. x. Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. We would like to show you a description here but the site won’t allow us. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Selects either 96-well or 384-well plate for a stand-alone read. This can increase throughput and efficiency, thus saving time and space in the lab. 384 Well Optically Clear Tissue Culture Plate: Plate length: 127. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Plate formats. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Total volume (except deep. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. T = I/I 0 and %T = 100 (T). 과 같은 Parameter를 검사하여 평가할 수 있습니다. Fluorescence Microplate Reader Comparison Chart. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 96-, 384-, and 1536-well microplates. 2, prime the machine with CellTiter-Glo ® 2. Dual injectors for kinetic assays. 5 pM 2. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). Typical 4 min/ 384 2 min/384 8 min/1536 Throughput plate plate plate Fully enabled protocols = reduced setup time. Others models hold the sample in place using surface tension. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. 384-Well Microarray/High Sample Recovery Plate. In many cases these plates have the same dimensions, check the plate dimensions are the same as one of the plates listed above. Rating: 3. Inquiry Basket. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. x. In general microplate washers are designed to leave only 2 microliters of residue per well and operate with an accuracy of 3% CV per well and a uniformity of 4% CV across a plate. The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. Dimensions 128 x 86 x 15 mm. An industry-renowned instrument for monitoring GPCRs and ion channels. –Min. Catalog number: AB1384W. All stand-alone instrument functions can be controlled using the software. Gemini EM Reader scans. Using Molecular Devices microplate readers and SoftMax. Devices’ StakMax® Microplate Stacker in a matter of minutes and . 5. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. #2704174, 2705402, 2705412, 2704180; Drummond Digital Microdispenser cat. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. QC tools and IQOQ services. This is equivalent to having 511 built-in filters, 384-well: 0. Fluorescence Microplate Reader Comparison Chart. 3% in white 96- and 384-well microplates Time. Source plate capacity; 1 x plates. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. 384-well TC plate, Poly-d-Lysine, Flat Clear. Dilution series for ZsGreen (green), AcGFP (blue) and DsRed (red) transfected cells. High base plate design is compatible with automation and for bar coding. For more sample throughput, the system canThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 384-well SBS microplates are designed with corners cut at a1 and h1 positions to conform to SBS nomenclature. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Add 100 µl (96-well plate) or 25 µl (384-well plate) of cell suspension to each well of poly-D-lysine coated plates. 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. A = 2 - log 10 (%T). Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. In addition, SoftMax Pro SoftwareAfter incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. MultiWash+ Microplate Washer. To ensure accurate cell numbers for the cell standard curves, cells were assayed immediately. 20 attomol ATP per well. The plate was mixed gently for 2 minutes on a plate shaker and then allowed to incubate at room temperature for 10 minutes to stabilize luminescence. . Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. To update the driver. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications. Figure 6. 8 mm distance between well bottoms to plate bottomValidated by Molecular Devices for the FLIPR reader. With the FLIPR 384 , you get the sensitivity, data fidelity, and real-time, kinetic information of the FLIPR I system in a 384-well mode. * Note: Programming the washer with a final aspirate height of 4. The SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. Figure 1. Embla washers are durable and easy to maintain. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. Plus 384. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. and 25 μL of each concentration was added in triplicate to a 384-well solid white plate. For more sample throughput, both readers can be easily. 2. For more sample throughput, the system canabsorbance plate readers. Introduction. The system provides automation for up to 50 microplates for easy walkaway automation. Bioz Stars score: 86/100, based on 1 PubMed. The price of a microplate reader depends on the technical equipment and the number of measurement technologies that the device can measure. Microplates for applications that require high sample recovery rates. M2. Filter. 40266. Tunable wavelength selection / monochromator. Next-generation luminescence plate reader. com Check our website for a current listing of worldwide distributors. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. EMax. x. SLS & DLS: 2 µL quartz cuvettes 45 µL quartz cuvettes 4 mL generic glass cuvette DLS only:Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. x. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. B. 5 pM 384-well (50 µL) 6 pM 10 pM 1536-well (8 µL) 19 pM 25 pM 0200-7015 (LUM 384. These lab workhorses are used in drug discovery, research, bioassay validation, and. Incubate the plates at room temperature for 20 min before reading plate luminescence using a Paradigm plate reader (Molecular Devices) (see Note 14). The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. In the M2, M5 and Plus. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 4 decades . Click SoftMax Pro Software User Guide. Option 1: Use a plate. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Round well design with a clear flat bottom 2. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax Plus 384 Microplate Reader (Molecular Devices cat. 6-384 (96- and 384-well with pipetting) 96-well microplates. Four wash/rinse bottles are included and configurable for both 96- and 384. Microplate Readers Molecular Devices Spectramax M3 Multi Mode Microplate Reader. (40 µl maximum) Clear, white, or black. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. Temperature control. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. x. SpectraMax i3x. Reading Capabilities: cuvette or microplate. 96- and 384-well plates; Wavelength(s): 635 nm (classification), 532 nm (reporter) Citations:. 384-Well SBS Microplate, clear, square well, flat bottom, sterile, with cover, working volume 90 µl, packaged in sleeves of 10. SpectraMax® ABS Plus microplate reader can run both cuvette-based and microplate reader applications on the same instrument. Use standard plate unless you see your type listed. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. Figure 2-23: Bottle holder To install the bottle holder: 1. This enhanced capability. Another consideration, says Amouretti, is plate format. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). TNF standard curve. 030 fmol Fluorescence Polarization (FP). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. What is the dead volume in 96 and 384 well compound plates? Dead volume is 5 micro liters. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. The Standard Thermo Scientific 384-well plate, white, is compatible with the majority of 384 block PCR and sequencing instruments. Plate readers, also known as. Fluorescence Microplate Reader Comparison Chart. 00. 1. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Minimum volume and maximum throughput with 96- or. the Molecular Devices SPECTRAmax® 190 and 250 plate. (blue) and DsRed (red) transfected cells in 384-well plates, read from the bottom. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). Some have optional microplate readers, allowing emission scans in up to 384-well plates, for example. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to calculate the. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. Some instruments can handle only 96-well plates and lower, whereas others can read up to the ultrahigh throughput densities of 1,536-well plates. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. 7. Time-resolved phosphorescence. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Visible absorbance -. High base plate design is compatible with automated handling (gripping) and bar coding. smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader, spectramax i3x. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. It is more inert and reduces compound interference. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Service Contracts, Preventative Maintenance and. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Versa Max. 2. 384 wells, the SpectraMax iD3 reader. In addition, SoftMax Pro SoftwareThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. 1. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. What are the dimensions of the plate? Code. x. A2000 Full Wavelength Microplate Reader, 6 ~ 384 Well Plate, SHANGHAI PEIOU ANALYSIS INSTRUMENT CO. Outer wells aligned for proper viewing with FLIPR. The fluid surface in a 384-well is a deeply curved meniscus. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. Algorithms are optimized for accurate cell detection and. M5. Add the new plate. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. USA. As the wells of this plate are closer together, the light is more likely to stray into adjacent wells. FlexStation® 3 Multi-Mode MicroplateAlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. We also prefer that fluorescence signals in one well don’t bleed into the other, as this means less background and more precise data. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. Supplier: Molecular Devices. 0 out of 5 $ 8,495. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Modefor the SpectraMax Multi-Mode Microp late Reader, as well as all other Molecular Devices' microplate readers. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. Method 1. Features. SpectraMax M2 and M2e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. For more sample throughput, the system canThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Made from polypropylene with conical well profiles to. 5577. 384 well plates 6–384 wells 6–384 wells 6–384 wells 6–1,536 wells (fluorometry, TRF, luminometry, AlphaScreen assays). SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Devices PathCheck® Sensor—the only patented technologySpectraMax ® M5 Multi-Mode Microplate Reader (Molecular Devices) Gemini EM Microplate Reader (Molecular Devices). 2 OD at 200 nm; this decreased to 0. SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. Published Dec 16, 2020 | Updated Dec 16, 2020. Plate Formats: 6- to 384-well plates. Pro, Dual Read mode was selected. use as little as 2 µL samples with 24- or 64-well plates. X. MultiWash™+ Microplate Washer benefits include, walk-up usability, 4 wash and rinse. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Gemini EM Reader scans. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. explore more. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 100 fmol 384-well (75 µL) 0. After reading the ELISA plate on both readers, each standard curve was nearly identical. Contact our support team with your questions regarding Molecular Devices products and applications. We would like to show you a description here but the site won’t allow us. ©2010 Molecular Devices, Inc. 2. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Molecular Devices 大中华区 Email: info. plated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Can read 96 well plates (optional 384 well). Versa Max. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andSupplier: Molecular Devices. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. ". Fluorescent Polarization: Solid black plates, as above. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. It has a. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. 96/384 - For the SpectraMax 340PC384, press to select to read a 96-well plate or a 384- well plate. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. #4TI-0214) UltraVision Plate 96 (Brooks Life Sciences cat. A rinse program is included for quick and easy daily. The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. For fluorescence intensity assays, the SpectraMax® M2 and M2e units feature an. 3 µl). 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Gemini XPS Reader scans. SpectraMax® Plus 384 provides ultrafast, full spectral range. Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. 96- and 384-well. SpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. x. Microplate readers are commonly used in life science laboratories. Microseal 384-Well PCR Plates. Round well design with a clear flat bottom 2. M2. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. 2. C. Microplate is validated by molecular devices for the FLIPR reader. Compare specifications and request a quote from leading suppliers. (Component A, C) is sufficient for 1 plate (96-, 384-, 1536-well). 20 attomol ATP per well. They are also fully compatible with the FlexStation 3 Multi-Mode Microplate Reader. Reader SpectraMax® Paradigm® Multi-Mode Microplate Reader. 1536-well plates < 100 amol (384-well) 1 HTRF • Cisbio HTRF detection with optimized Xenon light source and . #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. 384-Deep Square Well Plates. For more sample throughput, the system can6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. 1 x plates. For more sample throughput, the system can384 Well Plate, supplied by Molecular Devices LLC, used in various techniques. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. SpectraMax ID5 Plate Reader (Molecular Devices). This eliminates the need for standard curves, and Flexibility to read a variety of plate formats. 20 µL (96-well plates) or 10 µL (384-well plates. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. To prevent evaporation, the microplate was covered with a qPCR seal. 4 Equipment. Determine concentration using the Beer-Lambert LawMolecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modes<2 fg/well for firefly luciferase in 96- and 384- well top read. 020 fmol 0. Technical specifications. x. San Jose, CA 95134. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. 96/384-well plate formats. x. Figure 3. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. 96-Deep Round or Square Well Plates. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Turn on Plate Reader and wait for it to complete its start-up routine. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. The equation that allows one to calculate absorbance from % transmittance is. by a photomultiplier tube (PMT) or charge-coupled device (CCD) present within the plate reader. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. for ELISAs and most cell-based assays. 2. Suggested plates are black. 1 pM 1536-well: 0. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. These robust readers have been placed in labs from Antarctica to the. For more sample throughput, the system canPlate Background OD, which was used for subsequent tests, was measured as follows. . #S1207. and the A1 corner of the plate adapter (Figure 1-2). Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 1 fmol luciferase per well. The drawing shows a standard microplate of 15 mm height covered with a lid. 0 out of 5 $ 14,499. Published Dec 16, 2020 | Updated Dec 16, 2020 Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum amount of DNA needed. Four wash/rinse bottles are included and configurable for both 96- and 384. The washer is engineered to be simple to maintain and use, including features that reduce clogging and noise. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of. 0. The MultiWash+ Microplate Washer is an automated washer that is configurable for both 96- and 384- well plates. Also refer to this Multimode Reader Comparison. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. 1. Use the provided 3. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. Features. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. 384-well plates • Automated rinse reduces clogging • Quiet and efficient. 96 deep well plate: packed in sleeves of 6 , 24 per case. Test up to 3. Molecular Devices Gemini XS Microplate Spectrofluorometer Details. Experience the identifiably different. Following the flushing of the machine as described in Section 3.